The particular incident that led to Macaulay's imprisonment, twice, by the colonial power was

A.

His criticism of British rule through the Lagos Daily News

B.

His constant opposition to the expansion of the British rule

C.

The humiliation of the British officials through his successful defense of Eleko in London

D.

The attempt to silence British officials through the platform of the NNDP

Correct answer is C

In retaliation to the successful defence of Eshugbayi Eleko as the rightful king of Lagos and the humiliation this brought to the British officials, Macaulay was jailed twice by the colonial power thereafter.